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| Registros recuperados : 17 | |
| 2. |  | LIMA, P. S. C.; SENA, J. A. D.; SILVA, J. R. da; LEMOS, M. V. F. Estabilidade segregacional do plasmidio pHT 409 em celulas da linhagem de Bradyrhizibium sp. SEMIA 6033. In: CONGRESSO NACIONAL DE GENETICA, 44., 1998, Aguas de Lindoia. Program e resumos... Aguas de Lindoia: SBG, 1998. Genetics and Molecular Biology, v. 21, n. 3, p. 217. Supplement. Resumo n. 14.| Biblioteca(s): Embrapa Meio-Norte. |
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| 4. | | FIGUEIREDO, C. S.; MARUCCI, S. C.; TEZZA, R. I. D.; LEMOS, M. V. F.; DESIDÉRIO, J. A. Caracterização do gene vip3A e toxidade da proteína Vip3Aa50 à lagarta-do-cartucho e à lagarta-da-soja. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 9, p. 1220-1227, set. 2013. Título em inglês: Characterization of the vip3A gene and toxicity of Vip3Aa50 protein to fall armyworm and velvetbean caterpillar.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 8. | | SEBASTIÃO, I.; LEMES, A. R. N.; FIGUEIREDO, C. S.; POLANCZYK, R. A.; DESIDÉRIO, J. A.; LEMOS, M. V. F. Toxicidade e capacidade de ligação de proteínas Cry1 e receptores intestinais de Helicoverpa armigera (Lepidoptera: Noctuidae). Pesquisa Agropecuária Brasileira, Brasília, DF, v. 50, n. 11, p. 999-1005, nov. 2015. Título em inglês: Toxicity and binding capacity of Cry1 proteins to Helicoverpa armigera (Lepidoptera: Noctuidae) intestine receptors.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 9. | | CURVÊLO, C. R. da S.; PEREIRA, A. I. A.; GRECCO, E. D.; POLANCZYK, R. A.; PRATISSOLI, D.; LEMOS, M. V. F.; ANDRADE, G. S. Controle microbiano de pragas do algodoeiro: um estudo envolvendo bacillus thuringiensis e trichoplusia ni (lepidoptera: noctuidae). In: CONGRESSO BRASILEIRO DO ALGODÃO, 7., 2009, Foz do Iguaçu. Sustentabilidade da cotonicultura brasileira e expansão dos mercados: anais. Campina Grande: Embrapa Algodão, 2009. 1 CD-ROM| Biblioteca(s): Embrapa Algodão. |
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| 10. | | GUIDELLI-THULER, A. M.; SENA, J. A. D.; ABREU, I. L.; DAVOLOS, C. C.; ALVES, S. B.; POLANCZYK, R. A.; VALICENTE, F. H.; LEMOS, M. V. F. Bacillus Thuringiensis: diversidade gênica em isolados Lepidoptera-específicos. Arquivos do Instituto Biológico, São Paulo, v. 73, n. 4, p. 411-420, out./dez. 2008.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 11. | | CRIALESI-LEGORI, P. C. B.; DAVOLOS, C. C.; LEMES, A. R. N.; MARUCCI, S. C.; LEMOS, M. V. F.; FERNANDES, O. A.; DESIDÉRIO, J. A. Interação de proteínas Cry1 e Vip3A de Bacillus thuringiensis para controle de lepidópteros-praga. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 2, p. 79-87, fev. 2014. Titulo em inglês: Interaction of Cry1 and Vip3A proteins of Bacillus thuringiensis for the control of lepidopteran insect pests.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 12. | | CASTELLANE, T. C. L.; CAMPANHARO, J. C.; COLNAGO, L. A.; COUTINHO, I. D.; LOPES, E. M.; LEMOS, M. V. F.; LEMOS, E. G. M. Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hidrocarbon substrate. In: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, n. 96, p. 361-369, 2017.| Biblioteca(s): Embrapa Instrumentação. |
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| 13. | | GUIDELLI, A. M.; THULER, R. T.; CÍCERO, E. A. S.; LEMOS, M. V. F.; BORTOLI, S. A. de; SENA, J. A. D. Efeito de isolados de Bacillus thurigiensis para traça-das-crucíferas Plutella xylostella (Lepidoptera: Plutellidae) In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 20., 2004, Gramado. Programa e resumos... Gramado: Sociedade Entomológica do Brasil, 2004. p. 294 Resumos. EN-1000.| Biblioteca(s): Embrapa Hortaliças. |
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| 14. | | MARUCCI, S. C.; FIGUEIREDO, C. S.; TEZZA, R. I. D.; ALVES, E. C. da C.; LEMOS, M. V. F.; DESIDÉRIO, J. A. Relação entre toxicidade de proteínas Vip3Aa e sua capacidade de ligação a receptores intestinais de lepidópteros-praga. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 50, n. 8, p. 637-648, ago. 2015. Título em inglês: Relationship between toxicity of Vip3Aa proteins and their binding capacity to intestine receptors of lepidopteran pests.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 16. | | ALVES, M. de C.; ROSSI, J. R.; RODRIGUES, M. G. F.; ALVEZ, E. C. da C.; FERREUDO, A. S.; LEMOS, M. V. F.; DESIDÉRIO, J. A.; FERNANDES, O. A. Identificação e caracterização de genes vip e cry coleóptero-específicos em isolados de Bacillus thuringiensis. Pesquisa agropecuária Brasileira, Brasília, DF, v. 46, n. 9, p. 1053-1060, set. 2011 Título em inglês: Identification and characterization of coleoptera?specific vip and cry genes in Bacillus thuringiensis isolates.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 17. | | VIEIRA, L. G. E.; ANDRADE, A. C.; COLOMBO, C. A.; MORAES, A. H. de A.; METHA, A.; OLIVEIRA, A. C. de; LABATE, C. A.; MARINO, C. L.; MONTEIRO-VITORELLO, C. de B.; MONTE, D. C.; GIGLIOTI, E.; KIMURA, E. T.; ROMANO, E.; KURAMAE, E. E.; LEMOS, E. G. M.; ALMEIDA, E. R. P. de; JORGE, E. C.; ALBUQUERQUE, E. V. S.; SILVA, F. R. da; VINECKY, F.; SAWAZAKI, H. E.; DORRY, H. F. A.; CARRER, H.; ABREU, I. N.; BATISTA, J. A. N.; TEIXEIRA, J. B.; KITAJIMA, J. P.; XAVIER, K. G.; LIMA, L. M. de; CAMARGO, L. E. A. de; PEREIRA, L. F. P.; COUTINHO, L. L.; LEMOS, M. V. F.; ROMANO, M. R.; MACHADO, M. A.; COSTA, M. M. do C.; SÁ, M. F. G. de; GOLDMAN, M. H. S.; FERRO, M. I. T.; TINOCO, M. L. P.; OLIVEIRA, M. C.; VAN SLUYS, M-A.; SHIMIZU, M. M.; MALUF, M. P.; EIRA, M. T. S. da; GUERREIRO FILHO, O.; ARRUDA, P.; MAZZAFERA, P.; MARIANI, P. D. S. C.; OLIVEIRA, R. L. B. C. de; HARAKAVA, R.; BALBAO, S. F.; TSAI, S. M.; MAURO, S. M. Z. di; SANTOS, S. N.; SIQUEIRA, W. J.; COSTA, G. G. L.; FORMIGHIERI, E. F.; CARAZZOLLE, M. F.; PEREIRA, G. A. G. Brazilian coffee genome project: an EST-based genomic resource. Brazilian Journal of Plant Physiology, v. 18, n. 1, p. 95-108, 2006.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 17 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/09/2012 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
FEDERICI, M. T.; PICCHI, S. C.; STUCHI, E. S.; FADEL, A. L.; LAIA, M. L.; LEMOS, M. V. F.; LEMOS, E. G. M. |
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Afiliação: |
MARÍA TERESA FEDERICI, INIA; SIMONE CRISTINA PICCHI, UNESP; EDUARDO SANCHES STUCHI, CNPMF; ANDRÉ LUIZ FADEL, ESALQ; MARCELO LUIZ LAIA, UFVJM; MANOEL VICTOR FRANCO LEMOS, UNESP; ELIANA GERTRUDES MACEDO LEMOS, UNESP. |
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Título: |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Electronic Journal of Biotechnology, 2012. |
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ISSN: |
0717-3458 |
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DOI: |
10.2225/vol15-issue3-fulltext-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increas... Mostrar Tudo |
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Palavras-Chave: |
Microarray. |
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Thesagro: |
Xylella Fastidiosa. |
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Thesaurus NAL: |
Citrus. |
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Categoria do assunto: |
-- |
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Marc: |
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260 $c2012
520 $aXylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCitrus
650 $aXylella Fastidiosa
653 $aMicroarray
700 1 $aPICCHI, S. C.
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700 1 $aLAIA, M. L.
700 1 $aLEMOS, M. V. F.
700 1 $aLEMOS, E. G. M.
773 $tElectronic Journal of Biotechnology, 2012.
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